Low Voltage SEM and Correlative Microscopy to Analyze Delicate Biological Material

Fluorescence and SEM correlative microscopy for nanomanipulation of subcellular structures

Nanomanipulation under scanning electron microscopy (SEM) enables direct interactions of a tool with a sample. We recently developed a nanomanipulation technique for the extraction and identification of DNA contained within sub-nuclear locations of a single cell nucleus. In nanomanipulation of sub-cellular structures, a key step is to identify targets of interest through correlating fluorescenc...

Scanning Electron Microscopy (SEM) analysis and biological control of Ixodes ricinus using entomopathogenic fungi

In the present study, pathogenicity of four native strains of Entomopathogenic fungus; Metarhizium anisopliae, was studied against adult stage of Ixodes ricinus. For this purpose a total number of 180 adult ticks were examined in triplicate. Thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. Samples were incubated in separate Pet...

how to prepare biological samples and live tissues for scanning electron microscopy (sem)

in this article we review the application and procedures involved in scanning electron microscope (sem) to observe biological and live tissues through using sem at high resolution. we discuss practical methods for optimizing tissue preservation to achieve the two principal goals of biological specimen preparation: (a) preserving biological structures as close to their living configuration as po...

Application of Ionic Liquid on Biological Samples in Correlative Optical Microscopy and Scanning Electron Microscopy

Correlative optical microscopy and scanning electron microscopy (SEM) combines two techniques enabling the use both optical and electronic analysis techniques on the same sample. For example, optical techniques, such as fluorescence staining, fluorescence in situ hybridization (FISH) could in principle be used with SEM and energy dispersive spectroscopy (EDS) [1]. Optical microscopy provides ef...

Keysight Technologies Imaging Organic and Biological Materials with Low Voltage Scanning Electron Microscopy